pH is a critical parameter in method development and routine enzyme assay measurement. At the ideal pH value, called optimal pH, enzymes have their highest activity. Accurate and reliable ...

This article describes a simple method to determine the optimum pH for the activity of one enzyme in a coupled enzyme assay. Decoupling of the two-step reaction is achieved by heat denaturing the ...

pH is a measure of a solution's acidity or alkalinity. Acids have a pH below 7, releasing hydrogen ions when dissolved in water, while bases have a pH above 7, releasing hydroxide ions. Enzymes have ...

For the first time, researchers have successfully used computational simulations to modify an enzyme’s structure to increase the optimum temperature of a reaction. Researchers at Uppsala University ...