pH is a critical parameter in method development and routine enzyme assay measurement. At the ideal pH value, called optimal pH, enzymes have their highest activity. Accurate and reliable ...

This article describes a simple method to determine the optimum pH for the activity of one enzyme in a coupled enzyme assay. Decoupling of the two-step reaction is achieved by heat denaturing the ...

The factors that affect enzyme catalysis (that is, enzyme activity) will definitely affect the rate of enzymatic reactions, but the factors that affect the rate of enzymatic reactions do not ...

pH is a measure of a solution's acidity or alkalinity. Acids have a pH below 7, releasing hydrogen ions when dissolved in water, while bases have a pH above 7, releasing hydroxide ions. Enzymes have ...

For the first time, researchers have successfully used computational simulations to modify an enzyme’s structure to increase the optimum temperature of a reaction. Researchers at Uppsala University ...