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YouTubeTamilnadu Test House
@purelyzelal on Instagram: "Indole Test 🩷💛 The indole test is a biochemical test used to determine bacteria that are able to produce indole from the (tryptophan) using the tryptophanase enzyme. Bacteria is inoculated into tryptone broth (rich in tryptophan) and incubated for 24–48 hours at 35–37°C. When Kovac’s reagent is added to the broth culture, it reacts with indole to produce a red or pink ring at the top of the test tube. Positive bacteria 🩷: E.coli, proteus vulgaris, Morganella morgan
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Instagrampurelyzelal
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